Biosan Lab Results

Biosan Laboratories, Inc.

Test Results for Universal Bacteria Specialist, Inc.

Overview of the Test:

One biocide formulation was received for biocidal efficacy evaluation. A mixed bacterial inoculum was prepared and added to the test sample, along with a small amount of nutrient broth. The inoculated sample was incubated at room temperature. Sanicheck APB and Sanicheck SRB test kits were used for Acid Producing Bacteria (APB) and Sulfate Reducing Bacteria (SRB) detection.

Specifics of the Test:

Inoculum Organisms:

Staphylococcus aureus ATCC 6538 Escherichia coli ATCC 8739 Desulfovibrio desulfuricans  ATCC 7757

Inoculum Target:         1 x 106 cfu/mL Test Volume:                        100 mL

Sampling Times:         0, 2 hours, 4 hours, 7 days

Recovery Media:

Sanicheck APB Sanicheck SRB

Sample Identification:

Lab ID Sample ID
11139 Envirolyte O&G

 

Results: See Table 1.

Notes and Observations:

The Universal Bacteria Specialist Sample, Envirolyte O&G, showed complete reduction of both SRB and APB immediately after inoculation.

Alicia Davis

Laboratory Manager

Silveria Cristo

Microbiologist

 

Table 1. Biocide Efficacy

 

 

Sample ID

T = 0 T = 2 hours T = 4 hours T = 7 days
 

SRB (SRB/ml)

 

APB

 

SRB (SRB/ml)

 

APB

 

SRB (SRB/ml)

 

APB

 

SRB (SRB/ml)

 

APB

Control (sterile water) ≥ 105  

strong

≥ 105  

strong

≥ 105  

strong

≥ 104  

strong

Envirolyte O&G  

<10

 

no reaction

 

<10

 

no reaction

 

<10

 

no reaction

 

<10

 

no reaction